Journal: Experimental and therapeutic medicine

Article Title: PHLDA3 inhibition attenuates endoplasmic reticulum stress-induced apoptosis in myocardial hypoxia/reoxygenation injury by activating the PI3K/AKT signaling pathway.

doi: 10.3892/etm.2021.10045

Figure Lengend Snippet: Figure 4. Blockage of the p‑PI3K/AKT pathway inhibits the protective effects of PHLDA3 inhibition following H/R injury. (A) Cell Counting Kit‑8 assays were used to evaluate cell viability. (B) LDH release was detected by ELISA. (C) Apoptotic rate in each group. mRNA levels of ERS markers, including (D) CHOP, (E) GRP78 and (F) caspase‑12, were measured by reverse transcription‑quantitative PCR. Data are presented as the mean ± standard deviation. n=4/group. *P<0.05 vs. H/R + AdshPHLDA3 + PBS. #P<0.05 vs. H/R + AdshRNA + PBS. PHLDA3, pleckstrin homology‑like domain family A member 3; H/R, hypoxia/ reoxygenation; LDF, lactate dehydrogenase; GRP78, 78 kDa glucose‑regulated protein; AdshRNA, adenovirus encoding scrambled short hairpin RNA; AdshPHLDA3, adenoviral vectors encoding PHLDA3 shRNA; NS, not significant.

Article Snippet: Antibodies against p‐AKT (cat. no. 4060; 1:600), AKT (cat. no. 4691; 1:400) and CHOP (cat. no. 5554; 1:600) were purchased from Cell Signaling Technology, Inc. Horseradish peroxidase‐conjugated goat‐anti rabbit secondary antibodies were obtained from BIOSS.

Techniques: Inhibition, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation, shRNA

Journal: Experimental biology and medicine (Maywood, N.J.)

Article Title: Nucleated red blood cells participate in myocardial regeneration in the toad Bufo Gargarizan Gargarizan .

doi: 10.1177/15353702211013297

Figure Lengend Snippet: Figure 8. Biochemical assay results. At indicated days after cautery-injury (dac), samples of blood and/or the apical tissue were collected to analyze relevant substances by enzyme-linked immunosorbent assay or Western blotting. Data are presented as mean SD, n ¼ 3, respectively; *P < 0.05 and **P < 0.01 vs. Ctrl group were calculated by Dunnett’s post hoc test after ANOVA. (a). Apical TNF-a. (b). Apical IL1b. (c). Apical IL6. (d). Apical IL11. (e). Serum IL11. (f). Serum cardiotrophin-1 (CT-1). (g). Apical CT-1. (h). Serum erythropoietin (EPO). (i). Apical vascular endothelial growth factor (VEGF). (j). Serum VEGF. (k). Apical matrix metalloproteinase 2 (MMP2). (l). Apical MMP9. ADU: arbitrary densitometric unit relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

Article Snippet: The membrane was blocked in Odyssey Blocking Buffer (LI-COR Biosciences, Lincoln, NE, USA), then incubated overnight at 4 C in rabbit antihuman matrix metalloproteinase 2 (MMP2) Ab diluent (1:1000; cat. no. 4022, Cell Signaling Technology, Danvers, MA, USA) or in rabbit anti-human MMP9 Ab diluent (1:1000; cat. no. 2270, Cell Signaling Technology), and finally incubated in IR Dye 800CW-goat anti-rabbit IgG (1:10,000; cat. no. 926–32211, LI-COR Biosciences).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot